ZEB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88845
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0020.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0014.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Chromatin Immunoprecipitation Sequencing, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Chemotaxis, Chip Cytometry, Gel Supershift Assay, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EAEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGST
Reactivity Notes
Please note that Mouse was reported in PMID:35021086 which used a previous lot that is no longer available. The current lot of this antibody is not validated for Mouse. Please contact technical services if you have any questions.
Marker
Mesenchymal Cells Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ZEB1 Antibody
![Western Blot: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Western-Blot-NBP1-88845-img0021.jpg "Western Blot: ZEB1 Antibody [NBP1-88845]")
Western Blot: ZEB1 Antibody [NBP1-88845]
Western Blot: ZEB1 Antibody [NBP1-88845] - Mesenchymal-high cancers exhibit enhanced sensitivity to ferroptosis inducers. Representative cell lines showing enhanced sensitivity to GPx4 inhibitor ML162 correlates to low levels of E-cadherin, an epithelial marker. H1975 and A549: lung cancer; KLE and Ishikawa: endometrial cancer. Antibodies against E-cadherin, ZEB1, vimentin, and MTDH were used to detect protein extracts from non-small-cell lung cancer H1975 and A549, and endometrial cancer cell lines KLE and Ishikawa. Beta-Actin was detected as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746770/) licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88845-img0010.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP1-88845]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP1-88845]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP1-88845] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0008.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0011.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0015.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0017.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845]")
Applications for ZEB1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation Sequencing
Reported in scientific literature (PMID:35021086)
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Entrez Gene IDs
6935 (Human)
Gene Symbol
ZEB1
UniProt
Additional ZEB1 Products
Product Documents for ZEB1 Antibody
Product Specific Notices for ZEB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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