ZEB1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13159

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.00 mg/ml

View all ZEB1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an C-terminal portion of the human ZEB1 protein (between residues 1087-1124).

Localization

Nucleus and cytoplasm.

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZEB1 Antibody - BSA Free

Western Blot: ZEB1 AntibodyBSA Free [NBP2-13159]

Western Blot: ZEB1 AntibodyBSA Free [NBP2-13159]

Western Blot: ZEB1 Antibody [NBP2-13159] - Analysis of Zeb1 in (1) HeLa (2) Cos7 (3) HepG2 and (4) Ntera2 cell lysates.
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody - BSA Free [NBP2-13159]

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody - BSA Free [NBP2-13159]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP2-13159] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Simple Western: ZEB1 AntibodyBSA Free [NBP2-13159]

Simple Western: ZEB1 AntibodyBSA Free [NBP2-13159]

Simple Western: ZEB1 Antibody [NBP2-13159] - Lane view shows a specific band for ZEB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for ZEB1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:40

Simple Western

1:200

Western Blot

1:1000
Application Notes
In Western blot, a band is seen at ~180 kDa in HeLa cell lysate, cos7, Ntera2, and HepG2 cell lysates. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In ICC/IF, staining was seen in the nucleus with some weak punctate cytoplasmic staining in HeLa cells. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

1.00 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1, also known as AREB6, is a zinc finger transcription factor encoding protein that represses T-lymphocyte-specific interleukin-2 (IL-2) gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (PMID: 1840704). ZEB1 is expressed in both skeletal and heart muscle, but is not found in the liver. ZEB1 mutations can cause posterior polymorphous corneal dystrophy type 3 (PPCD3) as well as corneal dystrophy Fuchs endothelial type 6 (FECD6). The former is a condition characterized by the metaplasia and overgrowth of corneal endothelial cells thus leading to an increased risk for glaucoma. The latter ocular disorder, FECD6, leads to reduced vision and pain and is caused by loss of endothelium of the central cornea. ZEB1 antibodies are useful tools for studying certain degenerative ocular diseases.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Entrez Gene IDs

6935 (Human)

Gene Symbol

ZEB1

UniProt

P37275

Additional ZEB1 Products

Product Documents for ZEB1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ZEB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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