ZFP106 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74653

Novus Biologicals, part of Bio-Techne
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NB100-74653
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ZFP106 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1300 and 1350 of human zinc finger protein 106 using the numbering given in entry NP_071918.1 (GeneID 64397).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZFP106 Antibody

Western Blot: ZFP106 Antibody [NB100-74653]

Western Blot: ZFP106 Antibody [NB100-74653]

Western Blot: ZFP106 Antibody [NB100-74653] - Detection of Human and Mouse ZFP106 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells, and whole cell lysate (50 ug) from Jurkat, Ramos, and Mouse CT26.WT cells. Antibody: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: ZFP106 Antibody [NB100-74653]

Immunoprecipitation: ZFP106 Antibody [NB100-74653]

Immunoprecipitation: ZFP106 Antibody [NB100-74653] - Detection of human ZFP106 by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for IP at 6 ug per reaction. ZFP106 was also immunoprecipitated by rabbit anti-ZFP106 antibody NB100-74654. For blotting immunoprecipitated ZFP106, NB100-74653 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for ZFP106 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZFP106

Zinc finger protein homolog 106 (ZFP106) is a C2H2-type zinc finger protein that also contains 2 WD repeats. ZFP106 is highly expressed in striated muscle, brown fat, and developing brain and is regulated by myogenin and nuclear respiratory factor-1 (NRF-1). ZFP106 localizes to the nucleus in association with nucleolar transcriptional machinery. The WD40 repeat in ZFP106 appears to facilitate targeting of ZFP106 to the nucleolus and to mediate the interaction between ZFP106 and testis-specific gene 118 (TSF118). A two-hybrid screen has identified testis-specific Y-encoded-like protein (TSPYL) as a ZFP106 interacting protein. These data suggest an important role for ZFP106 in testis development.

Alternate Names

DKFZp451A239, FLJ34610, SH3BP3, SH3-domain binding protein 3, Zfp-106, zinc finger protein 106 homolog, zinc finger protein 106 homolog (mouse), Zinc finger protein 474, ZNF474FLJ45841

Entrez Gene IDs

64397 (Human)

Gene Symbol

ZFP106

UniProt

NP_071918.1 (Human)

Additional ZFP106 Products

Product Documents for ZFP106 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZFP106 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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