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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for ZFYVE16 Antibody

Immunogen

The immunogen recognized by the antibody maps to a region between residue 825 to 875 of human Endosome-Associated FYVE Domain Protein using the numbering given in entry NP_001098721.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZFYVE16 Antibody

Western Blot: ZFYVE16 Antibody [NBP2-36539]

Western Blot: ZFYVE16 Antibody [NBP2-36539]

Western Blot: ZFYVE16 Antibody [NBP2-36539] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Endofin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ZFYVE16 Antibody

Application
Recommended Usage

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZFYVE16

The ZFYVE16 gene encodes endofin, an endosomal protein implicated in regulating membrane trafficking. It is characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain positioned in the middle of the molecule (Seet et al., 2004 [PubMed 14613930]).[supplied by OMIM]

Alternate Names

DKFZp686E13162, Endofin, endosome-associated FYVE-domain protein, KIAA0305Endosome-associated FYVE domain protein, zinc finger FYVE domain-containing protein 16, zinc finger, FYVE domain containing 16

Gene Symbol

ZFYVE16

Additional ZFYVE16 Products

Product Documents for ZFYVE16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZFYVE16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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