ZHX2 Antibody (5E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00022882-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZHX2 Antibodies »

Product Specifications

Immunogen

ZHX2 (NP_055758, 691 a.a. ~ 788 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEEDLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEES

Reactivity Notes

Human. Other species not tested.

Localization

Nuclear

Specificity

ZHX2 - zinc fingers and homeoboxes 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ZHX2 Antibody (5E2)

Western Blot: ZHX2 Antibody (5E2) [H00022882-M01]

Western Blot: ZHX2 Antibody (5E2) [H00022882-M01]

Western Blot: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of ZHX2 over-expressed 293 cell line, cotransfected with ZHX2 Validated Chimera RNAi ( Cat # H00022882-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZHX2 monoclonal antibody (M01), clone 5E2 (Cat # H00022882-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunocytochemistry/ Immunofluorescence: ZHX2 Antibody (5E2) [H00022882-M01]

Immunocytochemistry/ Immunofluorescence: ZHX2 Antibody (5E2) [H00022882-M01]

Immunocytochemistry/Immunofluorescence: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of monoclonal antibody to ZHX2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ZHX2 Antibody (5E2) [H00022882-M01]

Immunohistochemistry-Paraffin: ZHX2 Antibody (5E2) [H00022882-M01]

Immunohistochemistry-Paraffin: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of monoclonal antibody to ZHX2 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml.
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Applications for ZHX2 Antibody (5E2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and transfected lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation, and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZHX2

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Alternate Names

Zona pellucida glycoprotein 2, zona pellucida glycoprotein 2 (sperm receptor), zona pellucida glycoprotein ZP2, Zona pellucida protein A, zona pellucida sperm-binding protein 2, Zp-2, ZPA

Entrez Gene IDs

22882 (Human)

Gene Symbol

ZHX2

OMIM

609185 (Human)

UniProt

NP_055758 (Human)

Additional ZHX2 Products

Product Documents for ZHX2 Antibody (5E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZHX2 Antibody (5E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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