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ZIC3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33207

Novus Biologicals, part of Bio-Techne
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NBP1-33207

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Mouse (99%), Xenopus (91%), Zebrafish (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZIC3. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZIC3 Antibody

Western Blot: ZIC3 Antibody [NBP1-33207]

Western Blot: ZIC3 Antibody [NBP1-33207]

Western Blot: ZIC3 Antibody [NBP1-33207] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:500
Immunocytochemistry/ Immunofluorescence: ZIC3 Antibody [NBP1-33207]

Immunocytochemistry/ Immunofluorescence: ZIC3 Antibody [NBP1-33207]

Immunocytochemistry/Immunofluorescence: ZIC3 Antibody [NBP1-33207] - Analysis of paraformaldehyde-fixed Human ESC, using antibody at 1:100 dilution.
Immunohistochemistry-Frozen: ZIC3 Antibody [NBP1-33207]

Immunohistochemistry-Frozen: ZIC3 Antibody [NBP1-33207]

Immunohistochemistry-Frozen: ZIC3 Antibody [NBP1-33207] - Frozen sectioned E13.5 Rat brain. Green: ZIC3 protein stained by ZIC3 antibody [N3C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for ZIC3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZIC3

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.

Long Name

Zic Family Member 3

Alternate Names

Heterotaxy 1, HTX1, ZNF203

Gene Symbol

ZIC3

Additional ZIC3 Products

Product Documents for ZIC3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZIC3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.