ZKSCAN3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31566
![Western Blot: ZKSCAN3 AntibodyBSA Free [NBP1-31566]](https://resources.bio-techne.com/images/products/ZKSCAN3-Antibody-Western-Blot-NBP1-31566-img0006.jpg "Western Blot: ZKSCAN3 AntibodyBSA Free [NBP1-31566]")
![Immunocytochemistry/ Immunofluorescence: ZKSCAN3 Antibody - BSA Free [NBP1-31566]](https://resources.bio-techne.com/images/products/ZKSCAN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31566-img0007.jpg "Immunocytochemistry/ Immunofluorescence: ZKSCAN3 Antibody - BSA Free [NBP1-31566]")
![Immunocytochemistry/ Immunofluorescence: ZKSCAN3 Antibody - BSA Free [NBP1-31566]](https://resources.bio-techne.com/images/products/ZKSCAN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31566-img0010.jpg "Immunocytochemistry/ Immunofluorescence: ZKSCAN3 Antibody - BSA Free [NBP1-31566]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ZKSCAN3. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ZKSCAN3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: ZKSCAN3 Antibody - BSA Free [NBP1-31566]
Immunocytochemistry/Immunofluorescence: ZKSCAN3 Antibody [NBP1-31566] - PLX4720 induces JNK2/p38 MAPK-dependent cytoplasmic translocation of ZKSCAN3. Representative confocal images of subcellular translocation of endogenous TFEB (green) and ZKSCAN3 (red) in A375 cells treated with PLX4720 (1 i1/4M, 12 h). n = 3 independent experiments. Image collected and cropped by Citeab from the following publication (Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun (2019) licensed under a CC-BY license.![Western Blot: ZKSCAN3 AntibodyBSA Free [NBP1-31566]](https://resources.bio-techne.com/images/products/ZKSCAN3-Antibody-Western-Blot-NBP1-31566-img0011.jpg "Western Blot: ZKSCAN3 AntibodyBSA Free [NBP1-31566]")
Western Blot: ZKSCAN3 AntibodyBSA Free [NBP1-31566]
Western Blot: ZKSCAN3 Antibody [NBP1-31566] - Western Blot: ZKSCAN3 Antibody [NBP1-31566] - Immunoblots for ZKSCAN3 in cytoplasmic and nuclear fractions of A375 cells treated with DMSO, PLX4720 (1 uM, 12 h), or Torin1 (1 uM, 3 h). Lamin B1 serves as the control for the nuclear fractions, whereas LAMP1 and Tubulin are the control of the cytoplasmic fractions. Note that PLX4720 induces cytoplasmic enrichment of ZKSCAN3, whereas Torin1 has no effect on its nucleocytoplasmic redistribution. Image collected and cropped by Citeab from the following publication (Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun (2019) licensed under a CC-BY license.![Immunohistochemistry-Paraffin: ZKSCAN3 Antibody - BSA Free [NBP1-31566]](https://resources.bio-techne.com/images/products/ZKSCAN3-Antibody-Immunohistochemistry-Paraffin-NBP1-31566-img0008.jpg "Immunohistochemistry-Paraffin: ZKSCAN3 Antibody - BSA Free [NBP1-31566]")
Immunohistochemistry-Paraffin: ZKSCAN3 Antibody - BSA Free [NBP1-31566]
Immunohistochemistry-Paraffin: ZKSCAN3 Antibody [NBP1-31566] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human lung using ZKSCAN3 antibody at 1:200 dilution. The primary antibody binding to ZKSCAN3 was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. Strong nuclear reactivity was observed.Applications for ZKSCAN3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ZKSCAN3
Alternate Names
dJ874C20.1, FLJ33906, Zf47, ZFP47, zfp-47, Zinc finger and SCAN domain-containing protein 13, Zinc finger protein 306KIAA0426, Zinc finger protein 309ZNF306ZFP306, Zinc finger protein 47 homolog, zinc finger protein with KRAB and SCAN domains 3, zinc finger protein zfp47, zinc finger with KRAB and SCAN domains 3, ZNF309, ZSCAN13
Entrez Gene IDs
80317 (Human)
Gene Symbol
ZKSCAN3
UniProt
Additional ZKSCAN3 Products
Product Documents for ZKSCAN3 Antibody - BSA Free
Product Specific Notices for ZKSCAN3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.