ZNF174 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86821

Novus Biologicals, part of Bio-Techne
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NBP1-86821
NBP1-86821-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZNF174 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VSGPQEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF174 Antibody

Western Blot: ZNF174 Antibody [NBP1-86821]

Western Blot: ZNF174 Antibody [NBP1-86821]

Western Blot: ZNF174 Antibody [NBP1-86821] - Analysis in control (vector only transfected HEK293T lysate) and ZNF174 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody [NBP1-86821]

Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody [NBP1-86821]

Immunocytochemistry/Immunofluorescence: ZNF174 Antibody [NBP1-86821] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & actin filaments.
Immunohistochemistry-Paraffin: ZNF174 Antibody [NBP1-86821]

Immunohistochemistry-Paraffin: ZNF174 Antibody [NBP1-86821]

Immunohistochemistry-Paraffin: ZNF174 Antibody [NBP1-86821] - Staining of human gallbladder shows moderate nuclear positivity in glandular cells.

Applications for ZNF174 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF174

ZNF174 is a transcriptional repressor. Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells.

Alternate Names

AW-1, zinc finger protein 174, ZSCAN8Zinc finger and SCAN domain-containing protein 8

Gene Symbol

ZNF174

Additional ZNF174 Products

Product Documents for ZNF174 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF174 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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