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ZNF217 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41378

Novus Biologicals, part of Bio-Techne
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NB100-41378

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KNAADDSADKVNKN corresponding to internal region according to NP_006517.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ZNF217 Antibody

Immunohistochemistry-Paraffin: ZNF217 Antibody [NB100-41378]

Immunohistochemistry-Paraffin: ZNF217 Antibody [NB100-41378]

Immunohistochemistry-Paraffin: ZNF217 Antibody [NB100-41378] - 3.8ug/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ZNF217 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in human prostate, spleen, testis and thymus lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115 kDa band according to NP_006517.1. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human prostate shows nuclear staning in select secretory cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZNF217

ZNF217 binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'

Alternate Names

DKFZp781O2147, KIAA0335, MGC125707, MGC125710, zinc finger protein 518, zinc finger protein 518A, ZNF518

Entrez Gene IDs

7764 (Human)

Gene Symbol

ZNF217

Additional ZNF217 Products

Product Documents for ZNF217 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF217 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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