ZNF217 Antibody

Western Blot: ZNF217 Antibody [NBP1-78189]

Western Blot: ZNF217 Antibody [NBP1-78189] - ZNF217, but not FTO, expression was induced by hypoxia in a HIF-dependent manner. MCF-7 and HCC-1954 subclones (upper panels), and ZR75.1 cells treated with vehicle or 200 nM digoxin (lower panels), were exposed to 20% or 1% O2 for 48 h and immunoblot assays were performed. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/11743), licensed under a CC-BY license.

Western Blot: ZNF217 Antibody [NBP1-78189]

Western Blot: ZNF217 Antibody [NBP1-78189] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-ZNF217 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZNF217 was also immunoprecipitated by rabbit anti-ZNF217 antibody NBP1-78188, which recognizes an upstream epitope.

Knockdown Validated: ZNF217 Antibody [NBP1-78189]

Knockdown Validated: ZNF217 Antibody [NBP1-78189] - MCF-7 subclones were exposed to 20% or 1% O2 for 48 h and immunoblot assays were performed. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/11743), licensed under a CC-BY license.