ZNF274 Antibody (4C12)

Novus Biologicals, part of Bio-Techne | Catalog # H00010782-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4C12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZNF274 Antibodies »

Product Specifications

Immunogen

ZNF274 (NP_598009, 420 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHT

Specificity

ZNF274 - zinc finger protein 274

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ZNF274 Antibody (4C12)

Western Blot: ZNF274 Antibody (4C12) [H00010782-M01]

Western Blot: ZNF274 Antibody (4C12) [H00010782-M01]

Western Blot: ZNF274 Antibody (4C12) [H00010782-M01] - Analysis of ZNF274 expression in transfected 293T cell line by ZNF274 monoclonal antibody (M01), clone 4C12.Lane 1: ZNF274 transfected lysate(74.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ZNF274 Antibody (4C12) [H00010782-M01]

Immunocytochemistry/ Immunofluorescence: ZNF274 Antibody (4C12) [H00010782-M01]

Immunocytochemistry/Immunofluorescence: ZNF274 Antibody (4C12) [H00010782-M01] - Analysis of monoclonal antibody to ZNF274 on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: ZNF274 Antibody (4C12) [H00010782-M01]

ELISA: ZNF274 Antibody (4C12) [H00010782-M01]

ELISA: ZNF274 Antibody (4C12) [H00010782-M01] - Detection limit for recombinant GST tagged ZNF274 is 0.1 ng/ml as a capture antibody.

Applications for ZNF274 Antibody (4C12)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24760766)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZNF274

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.

Alternate Names

GIOT2, GIOT-2, gonadotropin inducible transcription repressor-2, Gonadotropin-inducible ovary transcription repressor 2, gonadotropin-inducible transcription repressor 2, KOX7DKFZp434F1811, zinc finger protein 44, zinc finger protein 44 (KOX 7), Zinc finger protein 55DKFZp686L21136, Zinc finger protein 58ZNF504, Zinc finger protein KOX7, zinc finger protein ZnFP12, ZNF, ZNF55, ZNF58

Entrez Gene IDs

10782 (Human)

Gene Symbol

ZNF274

OMIM

605467 (Human)

UniProt

NP_598009 (Human)

Additional ZNF274 Products

Product Documents for ZNF274 Antibody (4C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF274 Antibody (4C12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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