ZNF281 Antibody (4F7)
Novus Biologicals, part of Bio-Techne | Catalog # H00023528-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4F7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ZNF281 (NP_036614, 787 a.a. ~ 893 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQS
Reactivity Notes
Human. Other species not tested.
Localization
Nuclear
Specificity
ZNF281 - zinc finger protein 281
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for ZNF281 Antibody (4F7)
![Western Blot: ZNF281 Antibody (4F7) [H00023528-M01]](https://resources.bio-techne.com/images/products/ZNF281-Antibody-4F7-Western-Blot-H00023528-M01-img0006.jpg "Western Blot: ZNF281 Antibody (4F7) [H00023528-M01]")
Western Blot: ZNF281 Antibody (4F7) [H00023528-M01]
Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 expression in transfected 293T cell line by ZNF281 monoclonal antibody (M01), clone 4F7.Lane 1: ZNF281 transfected lysate(96.9 KDa).Lane 2: Non-transfected lysate.![Western Blot: ZNF281 Antibody (4F7) [H00023528-M01]](https://resources.bio-techne.com/images/products/ZNF281-Antibody-4F7-Western-Blot-H00023528-M01-img0004.jpg "Western Blot: ZNF281 Antibody (4F7) [H00023528-M01]")
Western Blot: ZNF281 Antibody (4F7) [H00023528-M01]
Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 over-expressed 293 cell line, cotransfected with ZNF281 Validated Chimera RNAi ( Cat # H00023528-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF281 monoclonal antibody (M01), clone 4F7 (Cat # H00023528-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: ZNF281 Antibody (4F7) [H00023528-M01] - Detection limit for recombinant GST tagged ZNF281 is approximately 0.03ng/ml as a capture antibody.
Applications for ZNF281 Antibody (4F7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ZNF281
Long Name
Zinc Finger Protein 281
Alternate Names
GZP1, ZBP-99, ZNP-99
Entrez Gene IDs
23528 (Human)
Gene Symbol
ZNF281
UniProt
Additional ZNF281 Products
Product Documents for ZNF281 Antibody (4F7)
Product Specific Notices for ZNF281 Antibody (4F7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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