ZNF281 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76551

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ZNF281 Antibodies »

Product Summary for ZNF281 Antibody - BSA Free

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFP281. The immunogen is located within amino acids 600 - 650 of ZFP281. Amino Acid Squence: YSNKSESQKEDPFNIAEPR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF281 Antibody - BSA Free

Western Blot: ZNF281 AntibodyBSA Free [NBP1-76551]

Western Blot: ZNF281 AntibodyBSA Free [NBP1-76551]

Western Blot: ZNF281 Antibody [NBP1-76551] - Analysis in A-20 cell lysate with antibody at 0.5 ug/mL.

Immunocytochemistry/Immunofluorescence: ZNF281 Antibody [NBP1-76551] -

Immunocytochemistry/Immunofluorescence: ZNF281 Antibody [NBP1-76551] - A20 cells with ZFP291 antibody at 20 ug/mL.

Applications for ZNF281 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Western Blot

0.5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF281

ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.

Long Name

Zinc Finger Protein 281

Alternate Names

GZP1, ZBP-99, ZNP-99

Gene Symbol

ZNF281

UniProt

AAH51905 (Human)

Additional ZNF281 Products

Product Documents for ZNF281 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF281 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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