ZNF282 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81653

Novus Biologicals, part of Bio-Techne
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NBP1-81653
NBP1-81653-25ul
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZNF282 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DIAVYFSEDEWKNLDEWQKELYNNLVKENYKTLMSLDAEGSVPKPDAPVQAEPREEPCVWEQRHPEEREIPMDPEAGAEPLVPAQDASSQVKREDTLCVRGQRGLEERAIPTESITDSPISAQDLLSRIKQEEHQCVWDQQDLA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%). Reactivity reported in scientific literature (PMID: 25373738)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF282 Antibody

Immunocytochemistry/ Immunofluorescence: ZNF282 Antibody [NBP1-81653]

Immunocytochemistry/ Immunofluorescence: ZNF282 Antibody [NBP1-81653]

Immunocytochemistry/Immunofluorescence: ZNF282 Antibody [NBP1-81653] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653]

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653]

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653] - Staining of human prostate shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653]

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653]

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
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Applications for ZNF282 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF282

ZNF282 binds to the U5 repressive element (U5RE) of the human T cell leukemia virus type I long terminal repeat. It recognizes the 5'-TCCACCCC-3' sequence as a core motif and exerts a strong repressive effect on HTLV-I LTR-mediated expression

Alternate Names

HTLV-I U5 repressive element-binding protein 1, HTLV-I U5RE-binding protein 1, HUB1, zinc finger protein 282

Gene Symbol

ZNF282

Additional ZNF282 Products

Product Documents for ZNF282 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF282 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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