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ZNF35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30842

Novus Biologicals, part of Bio-Techne
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NBP2-30842
NBP2-30842-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QASSQQVHSENIKVWAPVQGLQTGLDGSEEEEKGQNISWDMAVVLKATQEAPAASTLGSYSLPGTLAKSEILETHGTMNFLGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF35 Antibody

Western Blot: ZNF35 Antibody [NBP2-30842]

Western Blot: ZNF35 Antibody [NBP2-30842]

Western Blot: ZNF35 Antibody [NBP2-30842] - Analysis in control (vector only transfected HEK293T lysate) and ZNF35 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ZNF35 Antibody [NBP2-30842]

Immunocytochemistry/ Immunofluorescence: ZNF35 Antibody [NBP2-30842]

Immunocytochemistry/Immunofluorescence: ZNF35 Antibody [NBP2-30842] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ZNF35 Antibody [NBP2-30842]

Immunohistochemistry-Paraffin: ZNF35 Antibody [NBP2-30842]

Immunohistochemistry-Paraffin: ZNF35 Antibody [NBP2-30842] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for ZNF35 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF35

ZNF35 may be involved in transcriptional regulation. Involved in cell differentiation and/or proliferation

Alternate Names

BRF2, Butyrate response factor 2, ERF2ERF-2, Protein TIS11D, RNF162Czinc finger protein 36, C3H1 type-like 2, TIS11DEGF-response factor 2, zinc finger protein 36, C3H type-like 1, zinc finger protein 36, C3H type-like 2, zinc finger protein, C3H type, 36-like 2

Gene Symbol

ZNF35

UniProt

Additional ZNF35 Products

Product Documents for ZNF35 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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