Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ZNF350 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human ZBRK1. The immunogen is located within amino acids 410 - 460 of ZBRK1. Amino Acid Squence: KRIHTREKQEAKKVENPPAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF350 Antibody - BSA Free

Western Blot: ZNF350 AntibodyBSA Free [NBP1-76344]

Western Blot: ZNF350 AntibodyBSA Free [NBP1-76344]

Western Blot: ZNF350 Antibody [NBP1-76344] - A-20 lysate with ZBRK1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Applications for ZNF350 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF350

ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.

Alternate Names

BERG36, BRF1zinc finger protein 36, C3H1 type-like 1, Butyrate response factor 1, cMG1, ERF1ERF-1, Protein TIS11B, RNF162BEGF-response factor 1, TIS11Bearly response factor Berg36, zinc finger protein 36, C3H type-like 1, zinc finger protein, C3H type, 36-like 1

Gene Symbol

ZNF350

UniProt

NP_067645 (Human)

Additional ZNF350 Products

Product Documents for ZNF350 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF350 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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