Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ZNF521 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521. The immunogen is located within the first 50 amino acids of ZNF521. Amino Acid Squence: KKRPEDGEELEDEAVHS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse: (88%)

Specificity

ZNF521 antibody is human specific.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZNF521 Antibody - BSA Free

Western Blot: ZNF521 AntibodyBSA Free [NBP2-41174]

Western Blot: ZNF521 AntibodyBSA Free [NBP2-41174]

Western Blot: ZNF521 Antibody [NBP2-41174] - Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: ZNF521 Antibody - BSA Free [NBP2-41174]

Immunocytochemistry/ Immunofluorescence: ZNF521 Antibody - BSA Free [NBP2-41174]

Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP2-41174] - Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: ZNF521 Antibody - BSA Free [NBP2-41174]

Immunocytochemistry/ Immunofluorescence: ZNF521 Antibody - BSA Free [NBP2-41174]

Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP2-41174] - Immunocytochemistry of ZNF521 (NT) in HeLa cells with ZNF521 (NT) antibody at 2.5 ug/mL.
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Applications for ZNF521 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2.5 ug/mL

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF521

Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes

Alternate Names

EHZF, Evi3, LIP3, ZFP521, zinc finger protein 263, Zinc finger protein FPM315, Zinc finger protein with KRAB and SCAN domains 12

Gene Symbol

ZNF521

Additional ZNF521 Products

Product Documents for ZNF521 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF521 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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