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ZNHIT3 Antibody (2F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00009326-M09

Novus Biologicals, part of Bio-Techne
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H00009326-M09

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ZNHIT3 (AAH17931, 1 a.a. ~ 155 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASLKCSTVVCVICLEKPKYRCPACRVPYCSVVCFRKHKEQCNPETRPVEKKIRSALPTKTVKPVENKDDDDSIADFLNSDEEEDRVSLQNLKNLGESATLRSLLLNPHLRQLMVNLDQGEDKAKLMRAYMQEPLFVEFADCCLGIVEPSQNEES

Specificity

Reacts with zinc finger, HIT type 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ZNHIT3 Antibody (2F8)

Western Blot: ZNHIT3 Antibody (2F8) [H00009326-M09]

Western Blot: ZNHIT3 Antibody (2F8) [H00009326-M09]

Western Blot: ZNHIT3 Antibody (2F8) [H00009326-M09] - Analysis of ZNHIT3 expression in HepG2 (Cat # L019V1).
Immunocytochemistry/ Immunofluorescence: ZNHIT3 Antibody (2F8) [H00009326-M09]

Immunocytochemistry/ Immunofluorescence: ZNHIT3 Antibody (2F8) [H00009326-M09]

Immunocytochemistry/Immunofluorescence: ZNHIT3 Antibody (2F8) [H00009326-M09] - Analysis of monoclonal antibody to ZNHIT3 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: ZNHIT3 Antibody (2F8) [H00009326-M09] - Detection limit for recombinant GST tagged ZNHIT3 is 0.3 ng/ml as a capture antibody.

Applications for ZNHIT3 Antibody (2F8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against cell lysate in western blot and recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZNHIT3

ZNHIT3/TRIP3 interacts with HNF-4 alpha, a transcription factor involved in glucose metabolism. As a protein associated with HNF-4, ZNHIT3/TRIP3 functions as a transcriptional coactivator. Alternate names for ZNHIT3/TRIP3 include Zinc finger HIT domain-containing protein 3, thyroid receptor-interacting protein 3, and HNF4a coactivator.

Alternate Names

HNF-4a coactivator, Thyroid hormone receptor interactor 3TR-interacting protein 3, Thyroid receptor-interacting protein 3, TRIP-3, TRIP3thyroid receptor interacting protein 3, zinc finger HIT domain-containing protein 3, zinc finger, HIT domain containing 3, zinc finger, HIT type 3, zinc finger, HIT-type containing 3

Entrez Gene IDs

9326 (Human)

Gene Symbol

ZNHIT3

Additional ZNHIT3 Products

Product Documents for ZNHIT3 Antibody (2F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNHIT3 Antibody (2F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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