ZW10 Antibody (2E5)

Novus Biologicals, part of Bio-Techne | Catalog # H00009183-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZW10 Antibodies »

Product Specifications

Immunogen

ZW10 (NP_004715, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. masfvtevla hsgrlekedl gtrisrltrr veeikgevcn miskkysefl psmqsaqgli tqvdklsedi dllksriese vrrdlhvstg eftdlkqqle

Reactivity Notes

Human. Other species not tested.

Specificity

ZW10 (2E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ZW10 Antibody (2E5)

Western Blot: ZW10 Antibody (2E5) [H00009183-M05]

Western Blot: ZW10 Antibody (2E5) [H00009183-M05]

Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5. Lane 1: ZW10 transfected lysatE (88.8 KDa). Lane 2: Non-transfected lysate.
Western Blot: ZW10 Antibody (2E5) [H00009183-M05]

Western Blot: ZW10 Antibody (2E5) [H00009183-M05]

Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi or non-transfected control. Blot probed with H00009183-M05. GAPDH (36.1 kDa) used as loading control.
ELISA: ZW10 Antibody (2E5) [H00009183-M05]

ELISA: ZW10 Antibody (2E5) [H00009183-M05]

ELISA: ZW10 Antibody (2E5) [H00009183-M05] - Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.

Applications for ZW10 Antibody (2E5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZW10

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase.

Alternate Names

E3 ubiquitin-protein ligase ZSWIM2, EC 6.3.2.-, MEKK1-related protein X, MEX, MGC33890, Zinc finger SWIM domain-containing protein 2, zinc finger, SWIM domain containing 2, zinc finger, SWIM-type containing 2, ZZZ2ZZ-type zinc finger-containing protein 2

Entrez Gene IDs

9183 (Human)

Gene Symbol

ZW10

OMIM

603954 (Human)

UniProt

NP_004715 (Human)

Additional ZW10 Products

Product Documents for ZW10 Antibody (2E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZW10 Antibody (2E5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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