ZWINT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82868

Novus Biologicals, part of Bio-Techne
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NBP1-82868
NBP1-82868-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZWINT Antibodies »

Product Summary for ZWINT Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZWINT Antibody

Western Blot: ZWINT Antibody [NBP1-82868]

Western Blot: ZWINT Antibody [NBP1-82868]

Western Blot: ZWINT Antibody [NBP1-82868] - Analysis in control (vector only transfected HEK293T lysate) and ZWINT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ZWINT Antibody [NBP1-82868]

Immunocytochemistry/ Immunofluorescence: ZWINT Antibody [NBP1-82868]

Immunocytochemistry/Immunofluorescence: ZWINT Antibody [NBP1-82868] - Staining of human cell line RH-30 shows localization to nucleoplasm, nuclear bodies & cytosol.
Immunohistochemistry-Paraffin: ZWINT Antibody [NBP1-82868]

Immunohistochemistry-Paraffin: ZWINT Antibody [NBP1-82868]

Immunohistochemistry-Paraffin: ZWINT Antibody [NBP1-82868] - Staining of human small intestine shows distinct cytoplasmic and membranous positivity in glandular cells.

Applications for ZWINT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZWINT

ZWINT encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

C20orf164, chromosome 20 open reading frame 164, dJ337O18.7, zinc finger SWIM domain-containing protein 3, zinc finger, SWIM domain containing 3, zinc finger, SWIM-type containing 3

Gene Symbol

ZWINT

Additional ZWINT Products

Product Documents for ZWINT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZWINT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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