Zyxin Antibody (4F9P3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16109

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16109-100ul
NBP3-16109-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4F9P3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Zyxin Antibodies »

Product Summary for Zyxin Antibody (4F9P3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Zyxin (Q15942). TQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Zyxin Antibody (4F9P3)

Western Blot: Zyxin Antibody (4F9P3) [NBP3-16109]

Western Blot: Zyxin Antibody (4F9P3) [NBP3-16109]

Western Blot: Zyxin Antibody (4F9P3) [NBP3-16109] - Western blot analysis of extracts of various cell lines, using Zyxin Rabbit mAb (NBP3-16109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (4F9P3) [NBP3-16109]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (4F9P3) [NBP3-16109]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (4F9P3) [NBP3-16109] - Immunofluorescence analysis of NIH-3T3 cells using Zyxin Rabbit mAb (NBP3-16109) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Zyxin Antibody (4F9P3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Zyxin

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Alternate Names

Zyx, Zyxin 2

Gene Symbol

ZYX

Additional Zyxin Products

Product Documents for Zyxin Antibody (4F9P3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Zyxin Antibody (4F9P3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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