Zyxin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46135

Novus Biologicals, part of Bio-Techne
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NBP1-46135
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Zyxin Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QQREKPRVQEKQH corresponding to internal region according to NP_003452.1.

Epitope

internal region

Specificity

Reported variants represent identical protein: NP_003452.1, NP_001010972.1

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Zyxin Antibody

Western Blot: Zyxin Antibody [NBP1-46135]

Western Blot: Zyxin Antibody [NBP1-46135]

Western Blot: Zyxin Antibody [NBP1-46135] - (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Zyxin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 85 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 61.3 kDa band according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Zyxin

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Alternate Names

Zyx, Zyxin 2

Entrez Gene IDs

7791 (Human)

Gene Symbol

ZYX

UniProt

NP_003452.1 (Human)

Additional Zyxin Products

Product Documents for Zyxin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Zyxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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