CellXVivo Mouse Dendritic Cell Differentiation Kit

Morphology of Mouse Dendritic Cells Derived from PBMCs. Mouse PBMCs were differentiated into immature mouse dendritic cells by culturing for 5 days in reagents included in the CellXVivo^™Mouse Monocyte-derived Dendritic Cells Differentiation Kit. Immature dendritic cells exhibit characteristic dendritic cell morphology.

Phenotypic Analysis of Cultured Immature and Mature Mouse Dendritic Cells. Immature dendritic cells (left, blue) and TNF-α-treated mature dendritic cells (right, red), derived from bone marrow cells cultured with the CellXVivo^™Mouse Dendritic Cell Differentiation Kit, were stained for flow cytometry with antibodies for CD11c, B7-2/CD86, MHC class II (I-A/I-E), or CD40 (open histograms). An appropriate isotype antibody (filled histograms) was used as a control. Immature dendritic cells have the phenotype of CD11c⁺, with intermediate expression of B7-2/CD86, MHC class II, and little to no CD40. Mature dendritic cells have the phenotype of CD11c⁺, with increased expression of B7-2/CD86, MHC class II, and CD40. All R&D Systems antibodies and corresponding catalog numbers used in this figure are highlighted below.

Kit-derived Mature Mouse Dendritic Cells Induce Proliferation of Allogeneic T Cells. Serial dilutions of TNF-α-treated mature dendritic cells were incubated with allogeneic mouse T cells for 3 days. 3 H-Thymidine (3 H-TdR) was added for the final 18 hours of the culture. Cells were harvested and the incorporation of 3 H-TdR was measured using a scintillation counter. Results are presented as the mean cpm of triplicates.