ExCellerate Human T Cell Expansion Media, Xeno-Free Best Seller

Fold Expansion of Human T Cells in ExCellerate Human T Cell Expansion Media. Primary human peripheral blood mononuclear cells (PBMCs) were cultured for 9 days in ExCellerate Human T Cell Expansion Media using activating beads and Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL). Cell counts were performed to determine fold expansion compared to the Day 0 seeding density (0.25 x 10⁶ cells/mL).

Phenotypic Analysis of Human T cells Expanded in Xeno-Free Human T Cell Expansion Media. Human PBMCs were cultured for 9 days in ExCellerate Human T Cell Expansion Media using activating beads and Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL). Human T Cells were enriched during the culture as indicated via the expression of positive T cell markers, including Mouse anti-Human CD3 epsilon APC-conjugated Antibody (Catalog # FAB100A) and with a Mouse Anti-Human CD8 alpha PE-conjugated Antibody (Catalog # FAB1509).

ExCellerate Human T Cell Expansion Media Enhances T Cell Expansion Compared to Other Commercial Media. 2.5 x 106 primary human peripheral blood mononuclear cells (PBMCs) were cultured for 9 days using ExCellerate Human T Cell Expansion Media or other commercially available media (Supplier #1 or #2), in combination with Recombinant Human IL-2 GMP (Catalog # 202-GMP), Recombinant Human IL-15 (Catalog # 247-GMP), Recombinant Human IL-7 GMP (Catalog # 207-GMP). The total number of T cells (A) and CD8+ T cells (B) were determined at days 5 and 9. ExCellerate Human T Cell Expansion Media shows superior expansion rates compared to other commercial media.

Performance of GMP Cytokines in ExCellerate Human T Cell Expansion Media. Primary human peripheral blood mononuclear cells (PBMCs) were cultured for 12 days in ExCellerate Human T Cell Expansion Media or another commercially available T cell media (Supplier #1) containing combinations of Recombinant Human IL-2 GMP (Catalog # 202-GMP), Recombinant Human IL-15 (Catalog # 247-GMP), Recombinant Human IL-7 GMP (Catalog # 207-GMP). The total number of T cells were quantified at days 0, 5, 8, and 12. Under all GMP cytokine conditions, T cells cultured using ExCellerate Human T Cell Expansion Media showed superior expansion compared to Supplier #1 media.

Superior Human T Cell Expansion Using ExCellerate Human T Cell Expansion Media and Plate-bound Anti-CD3 and Anti-CD28 Antibody Stimulation. Primary human peripheral blood mononuclear cells (PBMCs) were cultured for 15 days in ExCellerate Human T Cell Expansion Media or other commercially available media (Supplier #1 or #2), in combination with Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL) and plate-bound Mouse Anti-Human CD3 epsilon Antibody (Catalog # MAB100) and Human CD28 Antibody (Catalog # AF-342-PB). Fold expansion was determined and cell counts were performed at days 5, 11, and 15 to determine fold expansion compared to the Day 0 seeding density. ExCellerate Human T Cell Expansion Media shows superior expansion rates compared to other commercial media.

Lower Exhaustion Phenotype of T Cells Cultured Using ExCellerate Human T Cell Expansion Media Compared to Other T Cell Media. Primary human peripheral blood mononuclear cells (PBMCs) were cultured (0.4 x 10⁶ cells/mL) for 9 days using either ExCellerate Human T Cell Expansion Media or another commercially available T cell expansion media (Supplier 1). Both media were supplemented with Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL) and activating microbeads. (A) At days 5, 7, and 9, T cells fold expansion was determined determined. (B) T cells were resuspended in their respective media (0.4 x 10⁶ cells/mL), restimulated in culture with activating beads for an additional 6 days. At days 12 and 15 in culture, cells restimulated in ExCellerate Human T Cell Expansion Media showed continued expansion of T cells compared to the lower expansion rates of cell cultured in Supplier 1 media. (C) T cells were analyzed by flow cytometry for expression of PD-1, TIM3, LAG-1, and TIGIT, common surface markers of cell exhaustion. CD4+ T cells restimulated and cultured using ExCellerate Human T Cell Expansion Media showed low expression of cell exhaustion markers. Exhaustion markers on cells grown in Supplier 1 media show elevated expression levels.