GMP Cloudz Human T Cell Activation Kit GMP

Name: GMP Cloudz Human T Cell Activation Kit
Brand: R&D Systems
SKU: CLD001-GMP

R&D Systems, part of Bio-Techne | Catalog # CLD001-GMP

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Product Datasheet / COA / SDS

Dissolvable microspheres for the expansion of T cells under GMP conditions

Key Product Details

Features:

Dissolvable, magnetic-free Cloudz CD3/CD28 microspheres for the expansion of Human T-Cells ex vivo under GMP-grade conditions.

Key Benefits:

> 50-fold expansion of T cells from PBMCs cells in 10 days
Dissolvable CD3/28 microspheres enable T cell expansions
Optimized for use with the ExCellerate™ Human T Cell Media
Supports T cell expansion from PBMCs or purified CD3⁺ PBMCs
Optimized for use with R&D Systems cytokines and media
Cost Effective Approach for Immune Cell Activation

Discontinued Product

CLD001-GMP has been discontinued. View all Primary Cell Culture products.

Technical Data
Citations / Reviews

Summary for GMP Cloudz Human T Cell Activation Kit

GMP Cloudz Human T Cell Activation Kit

Why Expand T Cells ex vivo Using GMP Cloudz Human T Cell Activation Kit?

T Lymphocytes (T Cells) can be used as a powerful immunotherapy to fight against cancer and other diseases. GMP Cloudz Human T cell Activation Kit allows for a robust expansion platform utilizing dissolvable CD3/28 microspheres that can easily be removed from expanded cells. GMP Cloudz Human T Cell Activation kit supports the expansion of human T cell lines or T cell clones and the stimulation of human peripheral blood lymphocytes.

Versatility of GMP Cloudz Human T Cell Activation Kit

GMP Cloudz Human T Cell Activation Kit is optimized for T cell expansion when used in combination with ExCellerate Human T cell Expansion Media, Xeno-Free and GMP-Grade Proteins including IL-2, IL-7, and IL-15. The activation and cytokine/growth factor combinations used with this kit should be optimized by application or experimental protocol.

Kit Contents for GMP Cloudz Human T Cell Activation Kit

2 x 12 mL of GMP-Grade Cloudz CD3/CD28
2 x 100 mL of GMP-Grade Cloudz 6X Release Buffer
Each kit contains enough reagents to activate 4x10⁸ CD3⁺ T Cells.

Product Specifications for GMP Cloudz Human T Cell Activation Kit

Species	Human
Sterility Testing	Tested following USP <71> sterility guidelines.

Limitations

FOR RESEARCH AND PRECLINICAL OR EX VIVO CLINICAL USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

The safety and efficacy of this product in diagnostic uses has not been established.
This reagent should not be used beyond the expiration date indicated on the label.
Results may vary due to variations among cells derived from different donors.

Scientific Data Examples for GMP Cloudz Human T Cell Activation Kit

	Expansion of Human T Cells using GMP Cloudz™ Human T Cell Activation Kit Primary human CD3⁺ cells were activated with GMP Cloudz™ Human T-Cell Activation Kit and cultured for 9 days in ExCellerate™ T Cell Expansion Media (R&D Systems, Catalog # CCM030) and 20 ng/mL IL-2 (R&D systems, Catalog # 202-GMP). A. Cell counts were performed to determine fold expansion compared to the Day 0 seeding density (0.25 x 10⁶ cells/mL).
	Viability of Human T Cells using Cloudz™ Human T Cell Activation Kit. Primary human CD3⁺ cells were activated with GMP Cloudz™ Human T-Cell Activation Kit and cultured for 9 days in ExCellerate™ T Cell Expansion Media (R&D Systems, Catalog # CCM030) and 20 ng/mL IL-2 (R&D systems, Catalog # 202-GMP). Cell counts were performed to determine percent viability on days 5, 7, and 9 of the experiment compared to the Day 0 seeding density (0.25 x 10⁶ cells/mL).
	Phenotypic analysis of CD25/CD69 Markers Primary human CD3⁺ cells were A. activated with GMP Cloudz™ Human T Cell Activation Kit and cultured for 48 hours in ExCellerate™ T Cell Expansion Media (R&D Systems, Catalog # CCM030) and 20 ng/mL IL-2 (R&D systems, Catalog #202-GMP). B. The negative control was performed without adding Cloudz T Cell microspheres. Cells were fluorescently stained with using CD25-PE (R&D Systems, Catalog # FAB1020P) and CD69-a488 (R&D Systems, Catalog # FAB23591G) and analyzed via flow cytometry. Dead cells and debris were excluded from the analysis on scatter signals and viability dye. Cells that were treated with GMP Cloudz™ Human T Cell Activation Kit showed an activation signal 48 hours after reagent addition.

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the recommended temperature.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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