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Normal Goat Serum

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23475

Novus Biologicals, part of Bio-Techne
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NBP2-23475

Key Product Details

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Product Summary for Normal Goat Serum

Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Product Specifications

Host

Goat

Applications

Flow Cytometry (1:10 - 1:1000)

Application Notes

This product has been tested by SDS-PAGE and is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 26004262)

Formulation, Preparation, and Storage

Preparation Method

This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.

Purification

Multi-step

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide, 0.01% Gentamicin Sulfate

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Reconstitution Instructions

Reconstitute in 10 ml with deionized water.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Normal Goat Serum

Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

Alternate Names

10% NGS, blocking goat serum, blocking grade, NGS

Additional Normal Goat Serum Products

Product Documents for Normal Goat Serum

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Normal Goat Serum

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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