Lightning-Link (R) APC-Cy7 Antibody Labeling Kit
Novus Biologicals, part of Bio-Techne | Catalog # 765-0030
Key Product Details
Applications
Flow Cytometry
Kit Type
Antibody Labeling Kit
Conjugate
Allophycocyanin/Cy7
Product Summary for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit
Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).
The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates.
APC/Cy7 is a tandem conjugate. The APC is excited at 652nm and functions as an energy donor for the Cy7. Energy is transferred from the APC to the Cy7 via FRET (Fluorescence Resonance Energy Transfer). The Cy7 emits the energy received from the APC in the form of long wavelength light at 790nm
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay
The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates.
| Features | Benefits |
| Quick and easy to use | Save time, no special knowledge required |
| No separation steps | 100% recovery - no antibody/protein loss |
| Can be used in a wide range of applications | Flexible |
| Freeze dried | Ships at ambient temperature, long shelf-life |
| Fully scalable (10 ug to 1 g or more) | Easy transfer from R&D to manufacturing |
| Stringently QC tested | Consistent high quality, excellent batch-to-batch reproducibility |
| Large number of labels available | Experimental flexibility |
| Reliable: nearly 300 references | Successfully used in many fields of research |
APC/Cy7 is a tandem conjugate. The APC is excited at 652nm and functions as an energy donor for the Cy7. Energy is transferred from the APC to the Cy7 via FRET (Fluorescence Resonance Energy Transfer). The Cy7 emits the energy received from the APC in the form of long wavelength light at 790nm
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay
Product Specifications
Application Notes
Antibodies greater than 1mg/ml should be diluted to 1mg/ml using either milli-Q water or PBS. Antibodies less than 1mg/ml can still be used to generate good conjugates provided the maximum conjugation. Adding less than the recommended maximum amount of antibody may result in unbound label post conjugation. This kit is supplied with 3 vials, each suitable for labeling up to 15 ug of antibody.
Scientific Data Images for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit
Lightning-Link APC-Cy7 Antibody Labeling Kit [765-0030] - Mouse anti-human CD8 was conjugated with APC-Cy7 using an Expedeon Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).
Kit Contents for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit
- 1 or 3 glass vial(s) of Lightning-Link mix
- 1 vial of LL-Modifier reagent
- 1 vial of LL-Quencher reagent
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C.
Background: Lightning-Link (R) APC-Cy7
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
Additional Lightning-Link (R) APC-Cy7 Products
Product Documents for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit
Product Specific Notices for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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