What functional groups do I need on my protein?

Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.

Do I need to purify the conjugate?

No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.

Can non-antibody molecules be labeled?

Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.

Lightning-Link (R) APC Antibody Labeling Kit

Name: Lightning-Link (R) APC Antibody Labeling Kit
Brand: Novus Biologicals
SKU: 705-0015

Novus Biologicals, part of Bio-Techne | Catalog # 705-0015

Submit a review

Citations (43)

Product Datasheet / COA / SDS / Protocols & Manuals

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Applications

Flow Cytometry

Kit Type

Antibody Labeling Kit

Conjugate

Allophycocyanin

Product Summary for Lightning-Link (R) APC Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

Our APC antibody labeling kit enables the direct conjugation of APC to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link APC and incubates for 3 hours.

Features	Benefits
Quick and easy to use	Save time, no special knowledge required
No separation steps	100% recovery - no antibody/protein loss
Can be used in a wide range of applications	Flexible
Freeze dried	Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available	Experimental flexibility
Reliable: nearly 300 references	Successfully used in many fields of research

Allophycocyanin is a large 105kDa fluorescent phycobiliprotein. It absorbs at 652nm and emits at 666nm.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%.

Scientific Data Images for Lightning-Link (R) APC Antibody Labeling Kit

Flow Cytometry: Lightning-Link (R) APC Antibody Labeling Kit [705-0015]

Flow Cytometry: Lightning-Link APC Antibody Labeling Kit [705-0015] - Mouse anti-human CD8 was conjugated with APC using an Innova Biosciences Lightning-Link® kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).

Lightning-Link APC Antibody Labeling Kit [705-0015]

Kit Contents for Lightning-Link (R) APC Antibody Labeling Kit

1 or 3 glass vial(s) of Lightning-Link mix
1 vial of LL-Modifier reagent
1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) APC

Easy APC labeling of your antibody with 30 seconds hands-on time. Allophycocyanin (APC) is a large 105kDa fluorescent phycobiliprotein commonly used in FACS analysis. It absorbs at 650nm and emits at 662nm, but can be excited by either a dye laser (595-605nm), or a HeNe laser (633nm). The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Additional Lightning-Link (R) APC Products

Product Documents for Lightning-Link (R) APC Antibody Labeling Kit

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Lightning-Link (R) APC Antibody Labeling Kit

COA

SDS

Download SDS

Product Specific Notices for Lightning-Link (R) APC Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations
Reviews

Protocols
FAQs