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Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit

Novus Biologicals, part of Bio-Techne | Catalog # 370-0010

Novus Biologicals, part of Bio-Techne
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370-0010

Key Product Details

Kit Type

Antibody Labeling Kit

Conjugate

Biotin

Product Summary for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit

Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (See protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes).

Our Lightning-Link Rapid technology can be used to label small quantities of antibody with 100% recovery. The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates the reaction for only 15 minutes (for more details please watch the video below). As there are no desalting or dialysis steps that commonly interrupt traditional protein labeling procedures.

Features Benefits
Quick and easy to use Save time, no special knowledge required
No separation steps 100% recovery - no antibody/protein loss
Can be used in a wide range of applications Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more) Easy transfer from R&D to manufacturing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 references Successfully used in many fields of research


The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. Our biotinylation kits are optimised to create high quality conjugates and minimise assay background.

Lightning-Link Biotin Has been optimized for two separate applications: Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used. Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 3 vials, each suitable for labeling up to 200 ug of antibody.

Scientific Data Images for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit

Lightning-Link Rapid Type A Biotin Antibody Labeling Kit [370-0010] - CRP labeled with Lightning-Link Rapid Biotin A at 10ug, 100ug, 1mg, 10mg and 100mg single reaction vials tested with Streptavidin-Rabbit IgG in a sandwich ELISA. Detection: ABTS.

Kit Contents for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit

  • 1 or 3 glass vial(s) of Lightning-Link Rapid mix
  • 1 vial of LL-Rapid Modifier reagent
  • 1 vial of LL-Rapid Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) Rapid Type A Biotin

Additional Lightning-Link (R) Rapid Type A Biotin Products

Product Documents for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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