Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric)

Name: Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-38754

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38754

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

5.35 pg/mL (example only; lot dependent)

Assay Range

15.62 - 1000 pg/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric)

ELISA: Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric) [NBP3-38754]

ELISA: Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric) [NBP3-38754] - Standard Curve Reference

Kit Contents for Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 4-1BB Ligand/TNFSF9

CD137 exists on the cell surface as a monomer with a molecular mass of 30 kDa and as a dimer of 55 kDa. Human and mouse CD137 share 60% amino acid identity. CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen over stimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Gene Symbol

TNFSF9

Additional 4-1BB Ligand/TNFSF9 Products

Product Documents for Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human 4-1BB Ligand/TNFSF9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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