Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82443

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Acetylcholinesterase/ACHE ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Acetylcholinesterase/ACHE in samples. No Significant cross-reactivity or interference between Rat Acetylcholinesterase/ACHE and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.85

Inter-Assay Precision (Precision between assays) CV% < 4.74

Recovery for Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

Recovery

89-106%

Linearity

Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

Scientific Data Images for Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

ELISA: Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-82443] -

ELISA: Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric) [NBP2-82443] - Standard Curve Reference

Kit Contents for Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Acetylcholinesterase/ACHE

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Alternate Names

ACHE, ARACHE, N-ACHE

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Acetylcholinesterase/ACHE ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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