Rat ADAMTS13 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66441

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

View all ADAMTS13 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Rat

Specificity

This kit recognizes Rat ADAMTS13/VWFCP in samples. No significant cross-reactivity or interference between Rat ADAMTS13/VWFCP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.57%

Inter-Assay Precision (Precision between assays) CV% < 9.07%

Recovery for Rat ADAMTS13 ELISA Kit (Chemiluminescence)

Recovery

87-104%

Linearity

Rat ADAMTS13 ELISA Kit (Chemiluminescence)

Scientific Data Images for Rat ADAMTS13 ELISA Kit (Chemiluminescence)

Rat ADAMTS13 ELISA Kit (Chemiluminescence)

ELISA: Rat ADAMTS13 ELISA Kit (Chemiluminescence) [NBP2-66441] -

ELISA: Rat ADAMTS13 ELISA Kit (Chemiluminescence) [NBP2-66441] - Standard Curve Reference

Kit Contents for Rat ADAMTS13 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ADAMTS13

ADAMTS13 was first identified by its ability to cleave the ultra large aggregates of vWF into smaller forms in the serum. A thrombocytopenic disorder called thrombic thrombocytopenic purpura (TTR) was shown to be due to a deficiency in the cleavage of von Willebrands factor, and ADAMTS13 was identified as the enzyme responsible. ADAMTS13 cleaves the Tyr1605 to Met1606 bond in the A2 domain of vWF, a process stimulated by shear stress in the circulation and by binding to either platelet glycoprotein IBa or to heparin. The cleaved vWF leads to decreased platelet adhesion. An idiopathic form of TTR is found in patients with auto antibodies to ADAMTS13. The full length ADAMTS-1 sequence codes for a 1,427 amino acid protein, with a predicted mass of 153.6 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. The variants reported include 1,371, 1,340 and 344 amino acids, with predicted mass of 130.3, 144.5 and 36.7 kDa respectively. Many bands of varying sizes can be seen on Western blots, between 110-190 kDa, perhaps indicating differentially processed ADAMTS-13.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

Alternate Names

TTP, vWF-CP

Gene Symbol

ADAMTS13

Additional ADAMTS13 Products

Product Documents for Rat ADAMTS13 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat ADAMTS13 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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