Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66448

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.09 ng/mL

Assay Range

0.16-10 ng/mL

View all Adenylate Cyclase 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human ADCY1 in samples. No significant cross-reactivity or interference between Human ADCY1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.36%

Inter-Assay Precision (Precision between assays) CV% < 9.39%

Recovery for Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

Recovery

88-111%

Linearity

Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

ELISA: Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence) [NBP2-66448] -

ELISA: Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence) [NBP2-66448] - Standard Curve Reference

Kit Contents for Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adenylate Cyclase 1

Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Galpha s) for GTP, the dissociation of GTP-Galpha s from Gbetagamma and Galpha s)-mediated activation of adenylyl cyclase. Adenylyl cyclases type I (AC I) and III (AC III) have distinct staining within the cell nucleus of rat brain sensory neurons. AC I is expressed in myenteric ganglia as two bands of 160 kDa and 185 kDa by SDS-PAGE. Ca2+ stimulation of AC I and AC III is mediated by calmodulin. Protein associated with Myc (PAM) is a very potent inhibitor of AC I. A decrease in endogenous PAM levels in HeLa cells modulate both basal and agonist stimulated cAMP accumulation.

Long Name

Adenylate cyclase type 1

Alternate Names

ADCY1, Adenylyl cyclase 1, DFNB44, EC 4.6.1.1

Gene Symbol

ADCY1

Additional Adenylate Cyclase 1 Products

Product Documents for Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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