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Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77008

Novus Biologicals, part of Bio-Techne
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NBP2-77008-1Kit

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and cell culture supernates (50 - 100 uL)

Sensitivity

3.9 pg/mL

Assay Range

15.6 pg/mL - 1000 pg/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Canine

Specificity

This assay has high sensitivity and excellent specificity for detection of Canine Adrenomedullin/ADM. No significant cross-reactivity or interference between Canine Adrenomedullin/ADM and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

Recovery

82 - 110%

Scientific Data Images for Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

Canine Adrenomedullin/ADM ELISA Kit (Colorimetric) [NBP2-77008] -

Canine Adrenomedullin/ADM ELISA Kit (Colorimetric) [NBP2-77008] - Standard Curve Reference

Kit Contents for Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay Plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction Manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adrenomedullin/ADM

Adrenomedullin is a member of the calcitonin family of peptides and functions as a vasodilator. Adrenomedullin, a hypotensive peptide, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene related peptide. Human adrenomedullin mRNA was found to be highly expressed in several tissues. Adrenomedullain is secreted from a number of tissues including the adrenal medulla, endothelial and vascular smooth muscle cells. It has been implicated in a number of diseases including cardiovascular and renal disorders, sepsis, inflammation, diabetes and cancer.

Alternate Names

ADM

Gene Symbol

ADM

Additional Adrenomedullin/ADM Products

Product Documents for Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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