Human Agrin ELISA Kit (Chemiluminescence)

Name: Human Agrin ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-66661

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66661

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human AGRN in samples. No significant cross-reactivity or interference between Human AGRN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.7%

Inter-Assay Precision (Precision between assays) CV% < 9.28%

Recovery for Human Agrin ELISA Kit (Chemiluminescence)

Recovery

88-10%

Linearity

Scientific Data Images for Human Agrin ELISA Kit (Chemiluminescence)

ELISA: Human Agrin ELISA Kit (Chemiluminescence) [NBP2-66661] -

ELISA: Human Agrin ELISA Kit (Chemiluminescence) [NBP2-66661] - Standard Curve Reference

Kit Contents for Human Agrin ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Agrin

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Alternate Names

AGRN

Gene Symbol