Human Albumin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60093

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and urine (50 - 100 uL)

Sensitivity

0.019 ug/mL

Assay Range

0.078 ug/mL - 5 ug/mL

View all Albumin ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human albumin. No significant cross-reactivity or interference between human albumin and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Human Albumin ELISA Kit (Colorimetric)

Human Albumin ELISA Kit (Colorimetric)

Human Albumin ELISA Kit (Colorimetric) [NBP2-60093] -

Human Albumin ELISA Kit (Colorimetric) [NBP2-60093] - Standard Curve Reference

Kit Contents for Human Albumin ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-conjugate (100 x concentrate)
  • Biotin-conjugate Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Albumin

This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for human Albumin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated Albumin. A competitive inhibition reaction is launched between Albumin (Standards or samples) and biotin-conjugated Albumin with the pre-coated Albumin antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of Albumin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

ALB

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Human Albumin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Albumin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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