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Rat Albumin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60094

Novus Biologicals, part of Bio-Techne
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NBP2-60094-1Kit

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and urine (50 - 100 uL)

Sensitivity

0.01 ug/mL

Assay Range

0.01 ug/mL - 40 ug/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This assay has high sensitivity and excellent specificity for detection of rat MAU. No significant cross-reactivity or interference between rat MAU and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Rat Albumin ELISA Kit (Colorimetric)

ELISA: Rat Albumin ELISA Kit (Colorimetric) [NBP2-60094]

ELISA: Rat Albumin ELISA Kit (Colorimetric) [NBP2-60094]

ELISA: Rat Microalbunminuria ELISA Kit (Colorimetric) [NBP2-60094] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Rat Albumin ELISA Kit (Colorimetric)

Rat Albumin ELISA Kit (Colorimetric) [NBP2-60094] -

Rat Albumin ELISA Kit (Colorimetric) [NBP2-60094] - Standard Curve Reference

Kit Contents for Rat Albumin ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (100 x concentrate)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Albumin

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAU bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

ALB

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Rat Albumin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Albumin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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