Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric)

Name: Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-27198

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27198

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.236 U/L (example only; lot dependent)

Assay Range

0.625 - 40 U/L (example only; lot dependent)

View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric)

ELISA: Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric) [NBP3-27198] -

ELISA: Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric) [NBP3-27198] - Standard Curve Reference

Kit Contents for Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Gene Symbol

ALDH1A1

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric) Manual

COA

SDS

Download SDS

Product Specific Notices for Human Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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