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Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66416

Novus Biologicals, part of Bio-Techne
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NBP2-66416

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse ACTN1 in samples. No significant cross-reactivity or interference between Mouse ACTN1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.20%

Inter-Assay Precision (Precision between assays) CV% < 5.13%

Recovery for Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

Recovery

86-109%

Linearity

Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

ELISA: Mouse alpha-Actinin 1 ELISA Kit (Colorimetric) [NBP2-66416] -

ELISA: Mouse alpha-Actinin 1 ELISA Kit (Colorimetric) [NBP2-66416] - Standard Curve Reference

Kit Contents for Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha Actinin 1

Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Long Name

Actinin alpha 1

Alternate Names

ACTN1, alpha-Actinin 1, alphaActinin 1, BDPLT15

Gene Symbol

ACTN1

Additional alpha Actinin 1 Products

Product Documents for Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse alpha-Actinin 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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