Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82180

Novus Biologicals, part of Bio-Techne
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NBP2-82180
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

View all alpha-Galactosidase A/GLA ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human alpha-Galactosidase A/GLA in samples. No significant cross-reactivity or interference between Human alpha-Galactosidase A/GLA and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5%

Inter-Assay Precision (Precision between assays) CV% < 6%

Recovery for Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

Recovery

average = 95% for serum; average = 100% for EDTA plasma; average = 101% for cell culture media

Linearity

Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

Scientific Data Images for Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] -

ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] - Standard Curve Reference

Kit Contents for Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha-Galactosidase A/GLA

Galactosidase alpha encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Alternate Names

Agalsidase alpha, GALA, Melibiase

Gene Symbol

GLA

Additional alpha-Galactosidase A/GLA Products

Product Documents for Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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