Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68199

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all alpha-Methylacyl-CoA Racemase/AMACR ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human AMACR in samples. No significant cross-reactivity or interference between Human AMACR and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.51%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

Recovery

88-110%

Linearity

Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

Scientific Data Images for Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

ELISA: Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric) [NBP2-68199] -

ELISA: Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric) [NBP2-68199] - Standard Curve Reference

Kit Contents for Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents for Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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