Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66429

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes mouse ACTalpha2 in samples. No significant cross-reactivity or interference between Mouse ACTalpha2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.51%

Inter-Assay Precision (Precision between assays) CV% < 4.79%

Recovery for Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Recovery

92-109%

Linearity

Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Scientific Data Images for Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429]

ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429]

ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.
Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] -

ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - DRC image from verified customer review.
Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] -

ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.
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Kit Contents for Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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