Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68201

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all Amphiphysin/AMPH ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human AMPH in samples. No significant cross-reactivity or interference between Human AMPH and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.1%

Inter-Assay Precision (Precision between assays) CV% < 4.9%

Recovery for Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

Recovery

89-108%

Linearity

Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

Scientific Data Images for Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

ELISA: Human Amphiphysin/AMPH ELISA Kit (Colorimetric) [NBP2-68201] -

ELISA: Human Amphiphysin/AMPH ELISA Kit (Colorimetric) [NBP2-68201] - Standard Curve Reference

Kit Contents for Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Amphiphysin/AMPH

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Alternate Names

AMPH, AMPH1

Gene Symbol

AMPH

Additional Amphiphysin/AMPH Products

Product Documents for Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Amphiphysin/AMPH ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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