Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Name: Human AMPK alpha 1 ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-68257

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68257

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL

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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human AMPK in samples. No significant cross-reactivity or interference between Human AMPK and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.09%

Inter-Assay Precision (Precision between assays) CV% < 10.61%

Recovery for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Recovery

89-113%

Linearity

Scientific Data Images for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

ELISA: Human AMPK alpha 1 ELISA Kit (Chemiluminescence) [NBP2-68257] -

ELISA: Human AMPK alpha 1 ELISA Kit (Chemiluminescence) [NBP2-68257] - Standard Curve Reference

Kit Contents for Human AMPK alpha 1 ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: AMPK alpha 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol