Looking at item #NBP2-62857, what kind of plate reader would I use to read it? OD reader, fluoresnce plate reader

Name: Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-62857

The samples will fluoresce, so any plate reader that can detect fluorescence should be fine. You will need to measure the relative light units. See the manual for more information

Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62857

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Product Datasheet / COA / Protocols & Manuals

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

View all Angiopoietin-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Mouse

Specificity

This kit recognizes Mouse ANG1 in samples. No significant cross-reactivity or interference between Mouse ANG1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.76%

Inter-Assay Precision (Precision between assays) CV% < 8.76%

Recovery for Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)

Recovery

94-108%

Linearity

Scientific Data Images for Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)

ELISA: Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence) [NBP2-62857] -

ELISA: Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence) [NBP2-62857] - Standard Curve Reference

Kit Contents for Mouse Angiopoietin-1 ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol