Human Apelin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68234

Novus Biologicals, part of Bio-Techne
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NBP2-68234
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (50 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50 - 4000 pg/mL

View all Apelin ELISA Kits »

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human APLN in samples. No significant cross-reactivity or interference between Human APLN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.07%

Inter-Assay Precision (Precision between assays) CV% < 4.93%

Recovery for Human Apelin ELISA Kit (Colorimetric)

Recovery

85-102%

Linearity

Human Apelin ELISA Kit (Colorimetric)

Scientific Data Images for Human Apelin ELISA Kit (Colorimetric)

Human Apelin ELISA Kit (Colorimetric)

ELISA: Human Apelin ELISA Kit (Colorimetric) [NBP2-68234] -

ELISA: Human Apelin ELISA Kit (Colorimetric) [NBP2-68234] - Standard Curve Reference

Kit Contents for Human Apelin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apelin

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.

Alternate Names

AGTRL1 Lligand, APEL, APLN, XNPEP2

Gene Symbol

APLN

Additional Apelin Products

Product Documents for Human Apelin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Apelin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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