Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77030

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

19.5 ng/mL

Assay Range

78 ng/mL - 5000 ng/mL

View all Apolipoprotein A-I/ApoA1 ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Bovine

Specificity

This assay has high sensitivity and excellent specificity for detection of Bovine Apolipoprotein A-I/ApoA1. No significant cross-reactivity or interference between Bovine Apolipoprotein A-I/ApoA1 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Recovery

82 - 96%

Scientific Data Images for Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) [NBP2-77030] -

Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) [NBP2-77030] - Standard Curve Reference

Kit Contents for Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay Plate
  • HRP-Conjugate (100 x concentrate)
  • HRP-Conjugate Diluent
  • Instruction Manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bovine Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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