Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11735

Novus Biologicals, part of Bio-Techne
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NBP3-11735
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernatants (100 uL)

Sensitivity

0.2 ng/mL

Assay Range

0.63 - 40 ng/mL

View all Apolipoprotein A-I/ApoA1 ELISA Kits »

Product Specifications

Kit Type

ELISA Kit

Reactivity

Non-human Primate

Precision

Intra-Assay Precision (Precision within an assay) %CV < 6.0

Inter-Assay Precision (Precision between assays) %CV < 9.8

Recovery for Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit

Recovery

89 - 96%

Scientific Data Images for Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit

ELISA: Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit [NBP3-11735]

ELISA: Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit [NBP3-11735]

ELISA: Non-human Primate Apolipoprotein A-I/ApoA1 ELISA kit [NBP3-11735] - Representative standard curve.

Kit Contents for Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit

  • Biotinylated detection mAb (HDL 44)
  • Buffers for washing and dilution and standard reconstitution
  • Human apoA1 ELISA standard
  • Pre-coated ELISA strip plates (mAb HDL 110)
  • Standard reconstitution buffer
  • Streptavidin-HRP
  • Substrate (TMB)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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