Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18788

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

View all Apolipoprotein A-I/ApoA1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Apolipoprotein A-I/ApoA1 in samples. No significant cross-reactivity or interference between Rat Apolipoprotein A-I/ApoA1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.87

Inter-Assay Precision (Precision between assays) %CV < 5.21

Recovery for Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Recovery

89-108%

Linearity

Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

ELISA: Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) [NBP3-18788] -

ELISA: Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric) [NBP3-18788] - Standard Curve Reference

Kit Contents for Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Apolipoprotein A-I/ApoA1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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