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Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66737

Novus Biologicals, part of Bio-Techne
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NBP2-66737

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63-40 ng/mL

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Rat

Specificity

This kit recognizes Rat ApoE in samples. No significant cross-reactivity or interference between Rat ApoE and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.9%

Inter-Assay Precision (Precision between assays) CV% < 9.23%

Recovery for Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

Recovery

93-107%

Linearity

Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

Scientific Data Images for Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

ELISA: Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence) [NBP2-66737] -

ELISA: Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence) [NBP2-66737] - Standard Curve Reference

Kit Contents for Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein E/ApoE

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Alternate Names

APOE

Gene Symbol

APOE

Additional Apolipoprotein E/ApoE Products

Product Documents for Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Apolipoprotein E/ApoE ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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